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HS Code |
471125 |
| Strain Name | XL1-Blue |
| Species | Escherichia coli |
| Genotype | endA1 gyrA96(nalR) thi-1 recA1 relA1 lac [F′ proAB lacIqZΔM15 Tn10(TetR)] |
| Antibiotic Resistance | Tetracycline |
| Competence | Chemically competent |
| Reca Status | recA1 (recombination deficient) |
| Enda Status | endA1 (endonuclease deficient) |
| Lac Mutation | lacIqZΔM15 (enables blue/white screening) |
| Transformation Efficiency | 10^6 to 10^8 cfu/µg |
| Use Cases | Blue/white screening, high-efficiency cloning |
As an accredited Xl1 Blue E.Coli Cells factory, we enforce strict quality protocols—every batch undergoes rigorous testing to ensure consistent efficacy and safety standards.
| Packing | The packaging includes a small box containing 10 vials, each labeled “XL1-Blue E. coli Cells,” shipped on dry ice for preservation. |
| Shipping | XL1-Blue E. coli cells are shipped on dry ice to maintain viability and preserve their competent state. The cells should be stored immediately at –80°C upon arrival. Shipping includes insulated packaging to prevent temperature fluctuations and ensure the cells remain frozen throughout transit. Handle upon receipt with appropriate safety measures. |
| Storage | XL1 Blue E. coli cells should be stored at -80°C for long-term preservation. Keep the cells in sterile, tightly sealed cryovials to prevent contamination and desiccation. Avoid repeated freeze-thaw cycles, as this may reduce cell viability. When ready to use, thaw cells on ice and handle aseptically. For short-term storage, maintain at -20°C only if absolutely necessary. |
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Competency: Xl1 Blue E.Coli Cells with high transformation efficiency are used in plasmid cloning workflows, where rapid generation of recombinant DNA constructs is achieved. Selection Marker: Xl1 Blue E.Coli Cells with lacZΔM15 genotype are used in blue/white screening assays, where clear identification of recombinant colonies is enabled. Stability: Xl1 Blue E.Coli Cells with enhanced genomic stability are used in the propagation of unstable or repetitive DNA sequences, where high-fidelity plasmid maintenance is ensured. Contamination: Xl1 Blue E.Coli Cells with low endotoxin levels are used in DNA preparation for sensitive mammalian transfections, where reduced cytotoxicity and background noise is observed. Purity: Xl1 Blue E.Coli Cells with over 99% purity are used in high-throughput screening applications, where minimized genetic heterogeneity supports reproducible results. Antibiotic Resistance: Xl1 Blue E.Coli Cells containing tetracycline resistance are used in dual-antibiotic plasmid selection, where selective pressure ensures maintenance of both vectors. Growth Rate: Xl1 Blue E.Coli Cells displaying fast doubling time at 37°C are used in large-scale DNA production, where rapid biomass generation accelerates plasmid amplification. Storage: Xl1 Blue E.Coli Cells with cryopreservation stability at -80°C are used for long-term stock maintenance, where high post-thaw viability supports consistent downstream applications. Competence: Xl1 Blue E.Coli Cells chemically competent with >1x10^8 cfu/µg DNA efficiency are used in site-directed mutagenesis workflows, where high yield of desired mutants is delivered. Plasmid Yield: Xl1 Blue E.Coli Cells optimized for high plasmid copy number are used in maxiprep extractions, where increased plasmid DNA quantity simplifies downstream processing. |
Competitive Xl1 Blue E.Coli Cells prices that fit your budget—flexible terms and customized quotes for every order.
For samples, pricing, or more information, please call us at +8615371019725 or mail to admin@sinochem-nanjing.com.
We will respond to you as soon as possible.
Tel: +8615371019725
Email: admin@sinochem-nanjing.com
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