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HS Code |
653450 |
| Productname | Proline Endonuclease |
| Enzymeclass | Hydrolase |
| Ecnumber | 3.4.21.- |
| Specificity | Cleaves peptide bonds at proline residues |
| Molecularweight | Approximately 35 kDa |
| Optimalph | 7.5 |
| Optimaltemperature | 37°C |
| Sourceorganism | Recombinant E. coli |
| Formulation | Lyophilized powder |
| Storagetemperature | -20°C |
| Activityassay | Chromogenic substrate assay |
| Unitdefinition | One unit cleaves 1 µmol substrate/min |
| Cofactorrequirement | None |
| Purity | ≥95% by SDS-PAGE |
| Application | Peptide mapping |
As an accredited Proline Endonuclease factory, we enforce strict quality protocols—every batch undergoes rigorous testing to ensure consistent efficacy and safety standards.
| Packing | The Proline Endonuclease comes in a 1 mg vial, securely sealed, labeled with product details, storage conditions, and expiry date. |
| Shipping | Proline Endonuclease is shipped in insulated packaging with sufficient dry ice or cold packs to maintain temperature stability during transit. The enzyme is shipped via overnight or expedited service to ensure preservation of activity. Shipping includes appropriate safety and compliance documentation, and tracking information is provided upon dispatch. |
| Storage | Proline Endonuclease should be stored at –20°C in a tightly sealed container, protected from light and moisture to maintain stability and activity. Avoid repeated freeze-thaw cycles by aliquoting the enzyme. Store in a buffer recommended by the manufacturer, typically containing stabilizers like glycerol. Handle under aseptic conditions to prevent contamination and ensure long-term enzyme integrity. |
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Purity 98%: Proline Endonuclease purity 98% is used in recombinant protein analysis, where it enables high-specificity cleavage of proline-rich motifs. Molecular Weight 32 kDa: Proline Endonuclease molecular weight 32 kDa is used in targeted proteomics workflows, where its defined size allows efficient incorporation into enzymatic digestion protocols. Stability Temperature 37°C: Proline Endonuclease stability temperature 37°C is used in cell lysate processing, where thermal stability maintains consistent cleavage activity during incubation. Activity ≥1000 U/mg: Proline Endonuclease activity ≥1000 U/mg is used in peptide mapping assays, where high enzymatic activity ensures rapid and complete substrate digestion. Lyophilized Form: Proline Endonuclease lyophilized form is used in diagnostic kit formulations, where extended shelf life and easy reconstitution support large-scale assay deployment. Endotoxin Level <0.1 EU/µg: Proline Endonuclease endotoxin level <0.1 EU/µg is used in biotherapeutic development, where low endotoxin content minimizes immunogenicity risk in sensitive applications. Optimal pH 7.5: Proline Endonuclease optimal pH 7.5 is used in protein structural studies, where pH consistency preserves native protein conformations during enzymatic digestion. Nickase Activity: Proline Endonuclease nickase activity is used in genome editing workflows, where single-strand DNA cleavage introduces controlled nicks without causing double-strand breaks. |
Competitive Proline Endonuclease prices that fit your budget—flexible terms and customized quotes for every order.
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Tel: +8615371019725
Email: admin@sinochem-nanjing.com
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