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Agrobacterium Tumefaciens

    • Product Name Agrobacterium Tumefaciens
    • Alias Rhizobium radiobacter
    • Einecs 943-841-8
    • Mininmum Order 1 g
    • Factory Site Tengfei Creation Center,55 Jiangjun Avenue, Jiangning District,Nanjing
    • Price Inquiry admin@sinochem-nanjing.com
    • Manufacturer Sinochem Nanjing Corporation
    • CONTACT NOW
    Specifications

    HS Code

    747364

    Scientific Name Agrobacterium tumefaciens
    Organism Type Gram-negative soil bacterium
    Causes Disease Crown gall disease
    Mode Of Action Transfers T-DNA to plant cells
    Transformation Use Genetic engineering tool for plants
    Host Range Wide variety of dicotyledonous plants
    Optimal Temperature 25-28°C
    Oxygen Requirement Aerobic
    Motility Flagellated (motile)
    Shape Rod-shaped
    Colony Appearance White, smooth, circular colonies
    Antibiotic Resistance Generally resistant to rifampicin and sensitive to carbenicillin
    Storage Condition Store at 4°C for short-term, -80°C in glycerol stocks for long-term

    As an accredited Agrobacterium Tumefaciens factory, we enforce strict quality protocols—every batch undergoes rigorous testing to ensure consistent efficacy and safety standards.

    Packing & Storage
    Packing Sealed, sterile 50 mL vial with tamper-proof cap; clearly labeled “Agrobacterium tumefaciens, 1 x 10⁹ CFU/mL.” Biohazard warning.
    Shipping Shipping **Agrobacterium tumefaciens** requires temperature-controlled (4°C) packaging to maintain viability. The bacterial culture is securely sealed in leak-proof containers, labeled according to biosafety regulations. It is shipped with appropriate documentation, usually overnight or express courier, to minimize transit time and ensure culture integrity and safety during transportation.
    Storage Agrobacterium tumefaciens should be stored in a cool, dry place at 2–8°C when in liquid or plate culture form. For long-term storage, preserve in 15–20% glycerol stocks at –80°C to maintain viability. Ensure containers are tightly sealed, properly labeled, and protected from light. Always follow biosafety guidelines to prevent contamination and accidental release.
    Application of Agrobacterium Tumefaciens

    Purity 99%: Agrobacterium Tumefaciens with 99% purity is used in transformation of dicotyledonous plants, where high purity ensures optimal gene transfer efficiency.

    Optical Density (OD600) 0.8: Agrobacterium Tumefaciens with OD600 of 0.8 is used in agroinfiltration assays, where precise cell concentration supports uniform plant infection rates.

    Viability 95%: Agrobacterium Tumefaciens with 95% viability is used in binary vector delivery, where enhanced cell survival increases transformation success.

    Stability Temperature 4°C: Agrobacterium Tumefaciens stable at 4°C is used in long-term storage protocols, where cold stability maintains bacterial integrity for extended periods.

    Plasmid-Carrying Strain: Agrobacterium Tumefaciens carrying binary plasmids is used in transgenic crop development, where plasmid presence enables efficient DNA delivery to target genomes.

    Colony Forming Units (CFU) 1x10^9/mL: Agrobacterium Tumefaciens at 1x10^9 CFU/mL is used in leaf disc transformation, where high cell density improves transformation frequency.

    Molecular Weight 5 MDa: Agrobacterium Tumefaciens with 5 MDa Ti plasmid is used in plant genetic engineering, where large plasmid size facilitates transfer of complex gene constructs.

    pH Stability 6.5–7.5: Agrobacterium Tumefaciens stable at pH 6.5–7.5 is used in co-cultivation of explants, where pH stability supports vigorous bacterial growth.

    Antibiotic Resistance Marker: Agrobacterium Tumefaciens with kanamycin resistance is used in selection of transformed cells, where antibiotic marker enables effective screening of positive transformants.

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