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Acriflavine

    • Product Name Acriflavine
    • Alias Trypaflavine
    • Einecs 200-135-5
    • Mininmum Order 1 g
    • Factory Site Tengfei Creation Center,55 Jiangjun Avenue, Jiangning District,Nanjing
    • Price Inquiry admin@sinochem-nanjing.com
    • Manufacturer Sinochem Nanjing Corporation
    • CONTACT NOW
    VTB
    Specifications

    HS Code

    992652

    Name Acriflavine
    Chemical Formula C14H14ClN3
    Molecular Weight 259.74 g/mol
    Appearance Reddish-brown powder or crystals
    Solubility Soluble in water and ethanol
    Melting Point 206–209 °C
    Cas Number 8018-07-3
    Uses Antiseptic, antibacterial, antiviral
    Mechanism Of Action Intercalates into DNA, inhibiting replication
    Storage Conditions Store at room temperature, protected from light
    Ph Typically around 6-7 in aqueous solution
    Synonyms Triazine Yellow, Acriflavinium chloride
    Route Of Administration Topical or solution for external use
    Color Index Number 46025

    As an accredited Acriflavine factory, we enforce strict quality protocols—every batch undergoes rigorous testing to ensure consistent efficacy and safety standards.

    Packing & Storage
    Packing Acriflavine is packaged in a 25g amber glass bottle with a secure screw cap, labeled with hazard warnings and product details.
    Shipping Acriflavine is shipped in tightly sealed, chemically resistant containers to prevent leakage and degradation. It is transported as a hazardous material, following all safety regulations for handling and labeling. Packages are protected from light, moisture, and incompatible substances, ensuring safe delivery to laboratories or research facilities.
    Storage Acriflavine should be stored in a tightly closed, light-resistant container at room temperature (15–25°C), away from moisture and incompatible substances such as strong oxidizers. The storage area should be well-ventilated and clearly labeled. Protect from direct sunlight and sources of ignition. Keep out of reach of unauthorized personnel, following standard laboratory safety protocols for chemical storage.
    Application of Acriflavine

    Applications of Acriflavine in Industrial Manufacturing

    Acriflavine serves as a specialized chemical raw material with established performance in regulated industrial sectors. As a direct manufacturer, we supply consistent quality for high-value applications where regulatory approval, process stability, and product purity are essential. The following application scenarios are drawn exclusively from current and validated industrial practices, with details on standards, dosage, process integration, and finished product categories.

    1. Pharmaceutical Antiseptic Formulations

    This material remains an active ingredient in a range of non-sterile and topical antiseptic preparations for pharmaceutical use, particularly in wound disinfectant solutions and clinical irrigation fluids. The compliance landscape restricts its use to non-systemic applications, requiring careful dosage control per pharmacopeia guidelines and adherence to regional regulations for finished antiseptics. Downstream processors imbibe acriflavine during aqueous blending and filtration before bulk filling or sterile packaging. Quality control procedures evaluate antimicrobial consistency, absence of pyrogens, and color stability, which are critical for final market authorization.

    Industry compliance standards

    • European Pharmacopoeia (Ph. Eur.) monograph 2416 for acriflavine solution
    • British Pharmacopoeia (BP) standards on topical antiseptics
    • Good Manufacturing Practice (GMP) guidelines for non-sterile liquids (ICH Q7)
    • Regional registration: EU Biocidal Products Regulation (BPR)

    Typical usage ratio

    • 0.1–0.2% w/v in aqueous antiseptic solutions, formulated based on active ingredient requirements; final concentration must comply with the maximum limit set by each pharmacopoeia and regional legislation.

    Downstream process integration

    • Added during solution compounding in water-for-injection vessels
    • Subjected to microfiltration before bottling
    • Stabilization with pH control and preservatives (if applicable)

    Final product types

    • Wound irrigation fluids
    • Clinical skin disinfectant solutions
    • Veterinary topical antiseptics (non-systemic)

    2. Fish Farming Antimicrobial Treatments

    In aquaculture systems, acriflavine is a controlled additive used for the management of external protozoan and fungal infections in ornamental and breeding fish populations. These treatments address pathogens like Ichthyophthirius and Saprolegnia within regulated therapeutic windows. Downstream aquaculture chemical producers align with both animal health product standards and strict residue testing protocols. Accurate dilution and batch admixing are monitored to prevent overexposure, while the final products are supplied as ready-to-dilute or pre-dosed treatment packs for hatcheries and commercial fish farms.

    Industry compliance standards

    • EU Regulation (EC) No 1831/2003 on additives for animal feed (limited to non-food fish sectors)
    • National veterinary medicine registration bodies
    • ISO 9001 quality management for aquaculture inputs production

    Typical usage ratio

    • 1–2 mg/L in immersion baths (short duration, up to manufacturer-specified maximum); stock solutions generally prepared as 0.1% by weight, then diluted on-site

    Downstream process integration

    • Dissolved into aqueous bulk admixtures during batch compounding
    • Standardized dosing through automated filling lines
    • Integrated residue monitoring for regulatory compliance

    Final product types

    • Fish bath treatment concentrates for ornamental/aquarium use
    • Commercial hatchery disease prevention solutions (non-food fish only)

    3. Histological Staining Reagents in Diagnostic Laboratories

    Diagnostic reagent manufacturers employ this raw material within formulation of histological stains for use in microscopy, particularly as a nucleic acid marker and as a component in fluorescent staining protocols. Each batch must satisfy stringent analytical purity and contamination requirements per in vitro diagnostic regulations. Processors introduce acriflavine during concentrated dye solution production, followed by standardized bottle filling with UV-shielding and lot traceability. Documentation supports both research and regulated clinical environments, in which trace-level impurities can compromise result reliability.

    Industry compliance standards

    • ISO 13485 Medical Devices Quality Management (for IVD reagent manufacturing)
    • European In Vitro Diagnostic Medical Device Regulation (IVDR, 2017/746/EU)
    • United States FDA 21 CFR Part 820 (for clinical laboratory reagents requiring compliance)

    Typical usage ratio

    • 0.01–0.05% by weight in concentrated histology staining solutions; final dilution depends on target cell type and microscopy protocol, adjusted by downstream QC validation

    Downstream process integration

    • Added during liquid dye solution preparation and homogenization
    • High purity filtration and bottle filling in cleanroom environments
    • QC testing for fluorescence intensity and stability

    Final product types

    • Microscopy staining kits for cell and tissue analysis
    • Research-use-only (RUO) fluorescent dyes for nucleic acid visualization
    • Diagnostic kits for laboratory cytology and histology applications

    4. Surface Disinfectants for Medical and Laboratory Equipment

    The compound figures in specialized disinfectant preparations for non-critical medical devices and laboratory surfaces, where its antimicrobial attributes support hygiene regimes in high-risk environments. Manufacturers must comply with end-use biocide efficacy criteria and allowable exposure limits, in accordance with market exclusivity for surface-use concentrates. During blending, it is combined with other actives or surfactants, subjected to mixing, particle filtration, and aseptic packaging. Final blends are stability tested, and their antimicrobial spectrum is verified during batch release, ensuring readiness for facility-level disinfection protocols.

    Industry compliance standards

    • EN 13697: Chemical disinfectants and antiseptics—Quantitative non-porous surface test for product activity
    • European Biocidal Products Regulation (BPR, Regulation (EU) No 528/2012)
    • ISO 22716: Cosmetics—Good Manufacturing Practices, applied to cleaning agents in healthcare

    Typical usage ratio

    • 0.05–0.15% active by volume in ready-to-use surface disinfectant solutions, potentially up to 0.2% for high-contamination risk environments; ratios selected to balance efficacy and regulatory exposure limits

    Downstream process integration

    • Included during surfactant-disinfectant compounding stages
    • Homogenized in solution via high-speed mixing apparatus
    • Bottled in pre-sterilized containers for end-user dilution as needed

    Final product types

    • Hospital-grade surface disinfectant sprays
    • Laboratory bench decontamination fluids
    • Cleaning agents for non-critical medical equipment
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    Certification & Compliance
    More Introduction

    Acriflavine: Proven Performance in Modern Manufacturing

    Our Experience Brings Quality You Can Count On

    At our plant, we have been making Acriflavine for decades. Every batch starts with high-purity raw materials because you can’t fake quality. Over the years, we have improved the process, focusing on consistent particle size, bright color, and reliable purity. Our main model of Acriflavine, Acriflavine Hydrochloride, carries the CAS number 8063-24-9 and has become a standard choice for companies in research, pharmaceuticals, and certain industrial sectors. Each lot runs through a series of checks for appearance, content, and solubility. Consistency means researchers and technicians get results they can trust every time.

    Understanding What Sets Acriflavine Apart

    Ask anyone who has worked with disinfectants or biological stains for any length of time. There’s a reason they reach for Acriflavine. Its deep orange-red hue and dependable antibacterial properties have earned it a fixed place in labs and production lines. Our process focuses on minimum residue and maximum active content so you get a clean, reliable result in each application. While methylene blue and gentian violet compete as common stains and antiseptics, their activity spectrum and interaction with certain proteins set them on a different path than Acriflavine. Where gentian violet might overpower, Acriflavine offers sharp staining with less cell disruption. In our own production environment, safety remains front and center. Each step, from filtration to drying, is set up to protect workers and maintain the material’s natural fluorescence and stability.

    Dedicated to Daily Real-World Use

    We see Acriflavine as more than a substance with a chemical formula. It’s a trusted workhorse for disinfecting surfaces and treating minor wounds in veterinary medicine. In microbiology, it helps distinguish cellular structures and DNA. Manufacturing staff throughout our facility have seen firsthand how the right grade of Acriflavine—without unnecessary fillers—gives better results. We’ve worked alongside academic teams who depend on a bright, clear stain to make microscopic observations hold up to scrutiny in publication or regulatory review. With our Acriflavine, they can confirm results and maintain records with full confidence.

    Supporting Research and Industry Progress

    Some parts of the world rely on Acriflavine as a frontline agent against bacteria where other drugs don’t cut it. It’s one of the few compounds still trusted for topical applications, showing broad-spectrum activity against Gram-positive bacteria. We put in the work to maintain a controlled salt balance so the final product dissolves quickly and distributes evenly in solution. Chemical suppliers and researchers have told us that even slight shifts in manufacturing habits can cause unpredictable changes in dye intensity or clarity. We have learned that regular calibration of equipment and close inspection at every step pays off by preventing rework and downtime.

    Acriflavine Hydrochloride: Balance Between Safety and Performance

    Few products balance handling ease and biological activity like Acriflavine Hydrochloride. Thanks to its solubility in water and alcohol, it works across a variety of formulations, from simple wound washes to more complex pharmaceutical blends. Our plant follows GMP standards strictly—not by obligation, but because walking the line on quality keeps our customers returning. Acriflavine stands up well to light, so it sits stable on warehouse shelves longer than many alternatives. By adjusting pH and batch temperature, we fine-tune color and potency, sidestepping problems that lead to inactive end-products.

    Stepping Away from Commodity Chemicals

    Mass production sometimes means sacrificing personal touch. That’s not how we operate. Every order brings a chance to put our expertise into action, whether for a bulk shipment for a hospital group or a small, customized lot for a university study. Large labs have commented on how quickly our Acriflavine dissolves compared to generic stock. They tell us that saving five minutes on preparation means more time for analysis and less stress when deadlines loom. We don’t treat these comments lightly. Our factory runs pilot pre-mixes and solution stability tests with every formulation tweak. Each quality report matches samples from independent labs, so what goes to a customer is the same as our internal reference standard.

    Taking a Closer Look: Acriflavine vs. Other Dyes and Disinfectants

    Market shelves hold no shortage of antiseptics and microbiological stains. But experience reveals clear differences between Acriflavine and products such as crystal violet or basic fuchsin. Acriflavine maintains definition in slides without overpowering background elements, which matters for those long microscopy sessions. The buffer range is wide enough that technicians rarely see precipitation or fading. Our in-house trials have shown that Acriflavine maintains color after extended UV exposure, outlasting stains that degrade after a few days on a light table.

    We often answer questions about the differences between Acriflavine Hydrochloride and Acriflavine Neutral. Our feedback from veterinary clinics and agricultural researchers is that the hydrochloride form marries better with saline solutions, increasing comfort during field use. Industries needing robust, stable dyes lean toward our hydrochloride grade due to its resilience in mixed solvent applications. Basic fuchsin and gentian violet, while powerful, come with restrictions in many jurisdictions and lack Acriflavine’s broad-spectrum bacterial utility. That’s feedback direct from buyers and field researchers over the years, not just marketing talk. We share data with major institutions, giving them a fuller picture of dye behavior during routine application, so their teams experience fewer interruptions and less waste.

    Our Commitment to Safety and Environment

    Health standards have only grown stricter in recent years. Our team runs toxicity profiles and environmental impact assessments as an ongoing part of production. Worker safety comes first; we invest in ventilation, spill containment, and personal protective equipment beyond regulatory minimums. Waste management goes beyond filters and neutralizing tanks. We reclaim solvents, track rinsewater, and review every new process with a multi-disciplinary safety committee. No step is too small if it means protection for people and fewer surprises during audits. Proper labeling is a promise we keep through each barrel and bottle. Clients receive detailed documentation on content, batch testing, potential environmental risks, and preferred disposal methods. Downstream users, including clinics, know how to handle even trace residues. There’s no skipping the science here, since safety incidents can ripple across an entire supply chain. For us, environmental responsibility and product consistency go hand in hand.

    Responding to Shifts in Global Demand

    Pandemic years and shifting regulations taught us to adapt. When global supply chains tighten, reliable domestic Acriflavine manufacturing turns from a routine business into an essential service. Hospitals, research labs, and manufacturers call on us not only for top-grade product but also for real-time advice on storage and preparation. Early in the crisis, we prioritized the most time-sensitive sectors and coordinated with our logistics partners to keep distribution moving smoothly. Our plant invested in backup generator systems, new warehouse insulation, and on-the-ground quality assurance to cut delays. Being ready for the unknown comes down to disciplined recordkeeping and old-fashioned teamwork on the factory floor. In high-pressure seasons, our plant supervisors worked shoulder to shoulder with operators, not from behind glass. This hands-on style means we catch issues before orders leave our dock and keep our reputation for honesty solid. Customers trust our tracking system to show them stock locations, batch histories, and third-party inspection reports at a glance.

    Listening to End-User Feedback

    We never stop learning. Feedback loops from hospitals, food safety labs, and academia offer direct lessons for improvement. When a veterinary group said their staff needed a less dusty format, we tweaked our granulation process to reduce airborne particles. Students preparing slide samples asked for more vivid color—so we nudged our blending method to optimize dye uptake. There is no finish line in product improvement. Only last year, we increased the transparency of our product certification, making third-party test results accessible to customers before purchase. We take every technical question—no matter how minor—seriously. A question about shelf life can lead to another round of stability testing. Concerns about cross-compatibility with other disinfectants prompt formulation adjustments in our test labs. This open-door culture leads to small but important changes, like shifting to tamper-evident packaging seals and offering more precise dosing guidelines for new users.

    Finding Real-World Solutions for Daily Challenges

    Making Acriflavine isn’t just about raw output. Consistency, color strength, and safety trends drive how our plant evolves. Six years ago, large customers began requesting dye-free samples of our carry containers, reducing unintentional cross-contamination during transit. We moved quickly, working with suppliers to overhaul our packaging. By directly sourcing our solvents and refining raw input checks, we reduced total off-spec batches by nearly a third in the last decade. In direct applications, like wound care or surface disinfection, field staff need clear instructions and absolute product certainty. We meet with senior users to draft guides and deliver training materials built from real field experience. If customers need smaller, single-use packaging for clinics, we listen and adjust our pipeline accordingly. These changes might seem minor but, over time, lead to fewer customer complaints.

    Regulatory Experience and Compliance

    Our job includes more than manufacturing—it involves regulatory navigation. Acriflavine products may face regional approvals and use restrictions, especially in food-contact scenarios or where residue risk is a concern. We built expertise consulting directly with compliance officers at customer facilities. By planning audits ahead and studying country-specific guidelines, we reduce downtime, ensure continued availability, and keep customer businesses running smoothly. Our documentation never hides important details. Customers have full access to purity specs, solubility charts, and batch-by-batch analysis. Each year brings updates to transport, packaging, and end-of-life standards, and we keep clients informed. This transparency smooths the path for import-export, especially during global logistical disruptions.

    Looking Ahead

    As science and industry push forward, we adapt our lab and plant practices. Clients now ask about nanoscale staining and solubility in new blends. Our teams launched small pilot lines to evaluate how Acriflavine performs in innovative medical coatings and diagnostic kits. Feedback cycles are short, changes get tested directly, and lessons cross from factory floor to finished product in less time than before. We also recognize trends toward greener manufacturing. Biodegradability, low-VOC processing, and energy-saving methods are not buzzwords to us; they are ongoing investments. Acriflavine remains a key product, but it shares the stage as customers drive toward safer and cleaner workspaces.

    In Summary

    Producing Acriflavine is not just about chemical know-how. It’s experience, customer interaction, and a constant drive to improve. Whether it’s handling, purity, stability, or service, we commit in the same way our customers commit to their work. Acriflavine represents a meeting point—where tradition meets modern demands, where precision in the plant matches real-world needs, and where honest feedback shapes the product’s future. That is the approach we take and the guarantee we stand behind, every single day.