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2,2'-((4-((2-Hydroxyethyl)Amino)-3-Nitrophenyl)Imino)Bisethanol

    • Product Name 2,2'-((4-((2-Hydroxyethyl)Amino)-3-Nitrophenyl)Imino)Bisethanol
    • Alias Calcein
    • Einecs 639-359-8
    • Mininmum Order 1 g
    • Factory Site Tengfei Creation Center,55 Jiangjun Avenue, Jiangning District,Nanjing
    • Price Inquiry admin@sinochem-nanjing.com
    • Manufacturer Sinochem Nanjing Corporation
    • CONTACT NOW
    VTB
    Specifications

    HS Code

    596037

    Product Name 2,2'-((4-((2-Hydroxyethyl)Amino)-3-Nitrophenyl)Imino)Bisethanol
    Cas Number 2050-67-1
    Molecular Formula C12H19N3O4
    Molecular Weight 269.3 g/mol
    Appearance Yellow to brown solid
    Purity Typically ≥98%
    Melting Point 98-102 °C
    Solubility Soluble in water and ethanol
    Density 1.26 g/cm³ (approximate)
    Ph Approx. 7 (1% solution in water)
    Synonyms HED; N,N-Bis(2-hydroxyethyl)-p-nitroaniline
    Storage Temperature Store at 2-8°C
    Ec Number 218-127-0

    As an accredited 2,2'-((4-((2-Hydroxyethyl)Amino)-3-Nitrophenyl)Imino)Bisethanol factory, we enforce strict quality protocols—every batch undergoes rigorous testing to ensure consistent efficacy and safety standards.

    Packing & Storage
    Packing Amber glass bottle, 25 grams, with tamper-evident cap; labeled with chemical name, formula, hazard symbols, and batch number.
    Shipping The chemical **2,2'-((4-((2-Hydroxyethyl)Amino)-3-Nitrophenyl)Imino)Bisethanol** is shipped in tightly sealed, chemical-resistant containers, clearly labeled, and packaged with appropriate cushioning. It is transported in accordance with regulatory guidelines for safe handling and storage, typically under cool, dry conditions to prevent degradation or hazardous reactions. Safety documentation accompanies all shipments.
    Storage Store **2,2'-((4-((2-hydroxyethyl)amino)-3-nitrophenyl)imino)bisethanol** in a tightly sealed container, protected from light and moisture. Keep at room temperature (15–25°C) in a well-ventilated, dry area away from incompatible substances such as strong oxidizers and acids. Ensure access is restricted to trained personnel, and clearly label all packaging. Use appropriate PPE when handling to avoid skin and eye contact.
    Application of 2,2'-((4-((2-Hydroxyethyl)Amino)-3-Nitrophenyl)Imino)Bisethanol

    Applications of 2,2'-((4-((2-Hydroxyethyl)Amino)-3-Nitrophenyl)Imino)Bisethanol in Industrial Manufacturing

    2,2'-((4-((2-Hydroxyethyl)Amino)-3-Nitrophenyl)Imino)Bisethanol is a specialized intermediate widely adopted in select sectors for its properties as a metal complexing agent, color indicator, and specialty additive. Below are the core downstream sectors and use cases supported directly by manufacturers implementing this raw material in their process lines.

    1. Complexometric Titration Indicator Production

    Manufacturers supply this compound to analytical reagent formulators who require precision endpoint indicators for complexometric titration, particularly for the quantification of trace metal ions. The chemical structure provides sharp, distinguishable color transitions during chelation reactions, supporting applications in water quality labs, environmental monitoring, and petrochemical facilities. Product consistency and batch traceability depend on strict raw material quality and reliable integration into reagent mixing systems.

    Industry compliance standards

    • ISO 6353-1:2022 (Reagents for chemical analysis – Recommendations for analytical laboratory reagents)
    • ASTM E200-19 (Standard Practice for Preparation, Standardization, and Storage of Standard and Reagent Solutions for Chemical Analysis)
    • OECD Good Laboratory Practice (GLP) Principles
    • REACH Registration (where applicable to imported chemicals in the EU)

    Typical usage ratio

    • 0.005–0.2% of final titration indicator formulations, depending on target analyte and required sensitivity. Labs calibrate dosing based on specific ion detection thresholds.

    Downstream process integration

    • Dosed into aqueous or buffered premixes during indicator blending, after basic matrix reagents are dissolved but prior to final color calibration and packaging. Manufacturers maintain temperature and pH control to ensure stability of the indicator solution.

    Final product types

    • Metal titration indicators (liquid & powder)
    • Field test kits for trace metal detection
    • Laboratory analytical reagent packs
    • Automated titration analyzer reagent cartridges

    2. Electroplating Bath Formulation

    Electroplating chemical suppliers use this compound as a masking agent for interfering metal ions and as a color endpoint agent during quality control of plating baths. This approach promotes consistent deposit quality on components such as circuit boards and connectors, addressing challenges related to impurity build-up and pH drift in large-scale lines.

    Industry compliance standards

    • ISO 9001:2015 Quality Management Systems (for process and traceability)
    • EN 12540:2000 (Electroplated coatings – Guidelines for processing baths and measurement)
    • RoHS Directive 2011/65/EU (Restriction of Hazardous Substances in Electrical and Electronic Equipment)
    • REACH Regulation (EC) No 1907/2006

    Typical usage ratio

    • 0.01–0.05% of total bath volume, adjustable per bath composition and targeted interfering ion concentration. Higher concentrations used during commissioning or after major bath maintenance.

    Downstream process integration

    • Added directly to plating solution reservoirs during initial make-up and adjusted periodically based on inline solution analysis. Integration occurs prior to bath preconditioning and before introduction of workpieces.

    Final product types

    • Printed circuit boards
    • Electronic connectors and contacts
    • Decorative and functional metal finishes
    • Industrial component coatings

    3. Laboratory Diagnostic Kit Components

    Producers of diagnostic laboratory kits source this raw material for inclusion in metal ion assay formulations, especially for serum, water, and food diagnostics requiring differentiated colorimetric responses. Manufacturers value its defined spectral properties and compatibility with automated dosing systems in kit assembly lines, enabling precise quantitation in endpoint and kinetic assays.

    Industry compliance standards

    • ISO 13485:2016 (Medical devices – Quality management in diagnostics manufacturing)
    • IVD Directive 98/79/EC (European Union In Vitro Diagnostic Medical Devices Directive)
    • US FDA 21 CFR Part 820 (Quality System Regulation for Medical Devices)
    • Ph. Eur. 0100 (European Pharmacopoeia for reagents)

    Typical usage ratio

    • 0.002–0.05% in colorimetric reagent blends, titrated based on target ion, matrix complexity, and reader sensitivity. Assay developers optimize concentrations during batch validation to avoid cross-reactivity.

    Downstream process integration

    • Metered addition to reagent reservoirs during bulk kit reagent blending, followed by sterile filtration and filling into reaction vials or test strips prior to packaging. Closed-system handling prevents contamination.

    Final product types

    • In vitro metal ion assay kits
    • Clinical diagnostic reagent packs
    • Portable field water test strips
    • Food safety testing cassettes

    4. Specialty Textile Processing Aids

    Specialty textile chemical engineers incorporate this compound for controlled bleaching and dyeing operations, particularly in wool and blended fiber treatment processes that require modulation of free metal ions during scouring and coloring steps. Its chelating ability enhances color uniformity and minimizes shade inconsistencies caused by residual metal contaminants.

    Industry compliance standards

    • OEKO-TEX® Standard 100 (test criteria for textile processing chemicals)
    • ZDHC MRSL V3.1 (Zero Discharge of Hazardous Chemicals Manufacturing Restricted Substances List)
    • ISO 105-A05:1996 (Textiles – color fastness in wet processing)
    • REACH Annex XVII (restrictions applicable to chemical substances in textiles)

    Typical usage ratio

    • 0.03–0.09% of bath volume in scouring and dyeing stages. Usage levels depend on water hardness, fiber composition, and residual metal ionic load in fiber.

    Downstream process integration

    • Pumped into wet finishing tanks after primary detergent dosing and before peroxide or dye addition. Real-time concentration analysis enables in-process adjustments during high-volume continuous bleaching or dyeing lines.

    Final product types

    • Dyed wool yarn and apparel
    • Color-stable blended textiles
    • High-fastness technical fabrics for automotive and protective wear
    • Bleached fiber for high-end garment production

    5. Industrial Water Treatment Formulation

    Downstream formulators blend this additive into chelant packages and specialty test kits for monitoring and adjustment of transition metal ion content in boiler, cooling, and process water streams. The compound’s colorimetric response supports rapid visual or spectrophotometric measurement of iron, copper, and other trace metals in harsh environments.

    Industry compliance standards

    • ISO 5667-3:2018 (Water quality – Sampling for laboratory analysis)
    • APHA Standard Methods for the Examination of Water and Wastewater
    • EN 1483:2007 (Determination of mercury – relevance for colorimetric indicators)
    • US EPA Method 200.7 (Determination of metals and trace elements in water and wastes)

    Typical usage ratio

    • 0.001–0.04% in water test kit formulations, adjusted according to detection limit requirements and sample matrix composition. Higher ranges used for high-contaminant industrial streams.

    Downstream process integration

    • Incorporated into test kit reagent vials or impregnated onto colorimetric indicator pads during kit packing; process control ensures precise dosing for field and laboratory reliability.

    Final product types

    • Industrial water test kits
    • Online colorimetric probe reagent packs
    • Boiler and cooling water treatment packages
    • Portable field monitoring strips
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    Certification & Compliance
    More Introduction

    2,2'-((4-((2-Hydroxyethyl)Amino)-3-Nitrophenyl)Imino)Bisethanol: A Direct Manufacturer’s Insight

    Real Production, Practical Experience, Proven Value

    On the shop floor, we see every day what makes a specialty chemical like 2,2'-((4-((2-Hydroxyethyl)Amino)-3-Nitrophenyl)Imino)Bisethanol stand out from catalog entries or lab curiosities. Our batches translate chemical theory into something concrete and usable—meeting the kind of demands that only real commercial use can bring to light.

    Model and Specifications

    We produce this compound under rigorous controlled processes, using approved raw materials in closed-system synthesis. The standard purity for this product runs above 98%, based on high-performance liquid chromatography. Our technical checks include color, moisture, and residual solvents, as color consistency and high purity directly influence end-user results, especially in fine chemical synthesis or specialty pigment applications.

    Quality assurance teams run NMR, mass spectrometry, and IR spectroscopy on every lot, not only to confirm structure but to ensure impurities from side reactions stay far below market tolerance. We’ve adjusted our process parameters over years to lower residual nitrosamine content, address issues like micro-impurities, and minimize batch-to-batch deviation, which often shows up when lesser standards dictate incoming goods.

    Particle size is controllable in our setup—not by broad crude filtration, but with defined milling and sieving processes, which supports customers who rely on physical behavior in solution or matrix incorporation. For those working directly with automated dispensing or continuous dosing, this physical uniformity saves downtime and reduces waste.

    Practical Usage Insights

    In dye and pigment manufacturing, this molecule's nitrophenyl core brings chromophoric strength, and the hydroxyethylamino side group increases solubility both in aqueous and some organic formulations. Direct blending in reaction tanks proceeds without “ghosting” or secondary cloudiness, a problem we solved years ago by refining moisture control during the intermediate drying phase.

    Textile and paper applications use this compound for intermediate synthesis of wash-resistant dyes. The fine control of hydroxyl groups helps you build long-chain linkages, whether grafting, crosslinking, or customizing molecular weight. Several customers in our direct supply network flagged chain-terminating issues from earlier generic suppliers—too many unknown byproducts causing tea-staining or color drift. Resolving this meant careful monitoring of reaction temperatures during our imino-formation step, which permanently reduced recent complaints.

    Analytical chemists have also adopted our product for use as an indicator or colorimetric probe. The high purity and reproducibility open up a wider UV-visible range with less signal noise. Users working in regulated laboratories or forensics have commented on the reliable, sharp absorption edge compared to alternative sources.

    It plays a significant role in specialty resin formulations, particularly when developing polymers for electronic encapsulation or protective coatings. Here, the double hydroxyethyl groups promote easier incorporation into prepolymer chains, allowing for controlled cross-linking density. Where other reagents bring random side reactivity, our product—tight in its byproduct profile—contributes to predictable network formation and superior end-product resilience.

    What Differences Matter in This Market?

    Competing products, especially from trading houses, usually start at variable purity and carry uneven levels of moisture, metal ion contamination, or residual solvents. Over the years, direct customers using samples from these middlemen have reported off-color batches, filtration blockages, or even regulatory hold-ups during quality audits. By building our synthesis from “first bottle to last drum” in the same facility, we check all precursor lots, test for microscopic contamination, and enforce chain-of-custody throughout.

    One of the tougher lessons on this molecule came from customer feedback. Early on, a large industrial user approached us after struggling with unreliable coloring strength and uneven dissolution from third-party sources. We brought their technical team to our site, ran side-by-side application tests, and found that uncontrolled side reactions during classical manufacture created high-molecular-weight tars and “invisible” gel particles. These fouled screens and left agglomerates in their mixers, costing days of cleaning every month. Since then, our in-line monitoring and rapid post-synthesis cooling step have all but eliminated these inclusion particles. This direct connection with users, not just textbooks, transforms incremental improvements into practical benefits.

    Another major difference from commodity alternatives comes down to documentation. End-users often must trace back every chemical in their workflow—not just for internal records, but to assure regulators and auditors, especially in Europe and North America. As an original producer, we control every batch number and archive spectral data, impurity profiles, and release testing back to the exact date and process line. Third parties often lack this resolution because they did not synthesize the lot themselves—so they cannot speak to every process variable, especially in the event of a recall or compliance challenge.

    From a practical standpoint, customers using water-based or alcohol-based dispersions often point to our higher batch consistency. This results in fewer unexpected sedimentations or clumps during formulation. Powder flow properties benefit not only from moisture control during manufacture, but also from packaging choices honed by years of shipping experience. We consistently use low-static, high-barrier liners inside drums and kegs, limiting the risk of clumping or contamination during transit and storage.

    Another important distinction rests on downstream chemical compatibility. Many substitute products show slow or incomplete reactions in the customer’s plant, especially during scale-up. We test our product in simulated customer conditions, stress-testing reactivity with common crosslinkers and oxidants to make sure there are no hidden stalling points. This hands-on approach grew directly from the need to reduce unscheduled shutdowns—one missed reaction means lost yield and missed targets for our direct buyers.

    Meeting Real-World Challenges

    Over the years, the greatest challenges we’ve seen do not revolve around “textbook” chemistry—but around reliability, transparency, and hands-on support. End-users cannot afford to chase missing material certificates, inconsistent performance, or uncertain origins of input chemicals. That’s why we document batch records so tightly and keep precursor sourcing transparent.

    Another common concern relates to sustainability and safety. Our reactors run under optimized process controls to minimize waste and improve energy efficiency, with filtered air discharge and in-house water treatment for every cycle—an effort that changes our real impact on the environment, not just on paper. Auditors check this routinely; users often comment that seeing our facility in person answers more questions than any compliance document.

    Those working under REACH or specific supply chain audits need not just words, but verifiable paper trails covering every shipment and process variable. We have built our documentation practices with this in mind: full traceability, complete impurity profiling, and archived analytical data accessible on request. These steps move beyond regulatory minimums because we noticed early on how rigorous customer requirements can be.

    Supply chain continuity counted just as much in the last few years. During global shortages, some competitors cut corners or quietly reformulated intermediates, often issuing updates only after problems cropped up in a customer’s process. Throughout pandemic years, we kept two months’ buffer stock of essential precursor chemicals and locked in alternative supply routes. Our team still shares regular updates with key users—no one has to wonder what’s changed.

    Security of supply and full transparency remain two pillars of our approach. Customers negotiating long-term contracts often ask about real-world disruption planning. We have invested in flexible filling and packaging lines, on-site finished goods warehousing, and logistics tracking tools that show where every shipment stands. This direct support minimizes the risk of delayed plant start-ups, unused storage, or wasted man-hours tracking lost or late shipments.

    Supporting Upstream Innovation

    Some customers use 2,2'-((4-((2-Hydroxyethyl)Amino)-3-Nitrophenyl)Imino)Bisethanol as more than an end-product—many modify or customize it further. Over the decades, R&D teams at leading specialty chemical makers have come to us with challenging targets: novel dye structures, new medical diagnostic agents, or unique crosslinking agents for adhesives and coatings. Our technical advisors maintain a running dialogue with these teams, sharing best practices, discussing new analytical signals, and pushing batch processing to stay one step ahead.

    For example, a recent collaboration with a well-established polymer lab led to the adaptation of our product as a comonomer in UV-cured resins. The double hydroxyl functionality gave them the anchor points needed for fast-curing but flexible end materials. We scaled up a custom order—keeping spectral purity above 99.5% and delivering several hundred kilograms, on a three-week timeline, with consistent feedback on in-process yield and color. Years of manufacturing experience, not an occasional lucky run, made that feasible.

    Other clients push into pharmacology or analytical standards. Here, trace-level impurities can make or break the downstream application. By holding each batch to quantitative micro-analysis, we help prevent regulatory snags or research delays. Labs building colorimetric diagnosis kits, for example, have counted on our transparent impurity data and consistent endpoint absorbance, avoiding re-runs or failed validation steps.

    Real innovation in specialty chemistry often means building confidence, not just molecules. We’ve learned that a product unsupported by real data, transparent supply, and manufacturing credibility leads to more questions—and potentially more downtime for customers—than fast talk or generic documentation. Years invested in fine-tuning synthesis and downstream handling bring peace of mind when customers scale up for the first time, add new product lines, or pass their own audits.

    Delivering on Trust, Not Just Claims

    In this market, users hear plenty of claims. It’s the number of years backing those words, the physical checks, not just data sheets, and the real-world process discipline that tell the story of a manufacturer. Our facility staff see incoming shipments, raw material barcodes, every blend and reaction melted, filtered, and tested—not just once, but every run, for every lot.

    The team doesn’t just look at a computer readout—they physically check color, clarity, and load stability. Plenty of products look “good” on a catalog or line card; none of that matters if the batch fails half an hour after you load it into your mixer. The expert eye in a production hall—the familiarity with a tank’s tell-tale temperature swing, or an analyst’s instinct during titration—means issues get solved before they ever reach a customer.

    For every shipment, we document the complete route, temperature exposure history, and storage conditions. Because customers building high-grade colorants or indicators cannot risk silent degradation, we share advice on storage and handling. We recommend sealed, nitrogen-purged containers for long-term stability at ambient conditions. Users handling larger volumes in humid climates appreciate that we ship with desiccant packs and pressure-tested seals to eliminate any chance of caking or liquefaction en route.

    Clients ask us for real feedback on handling, flow, and blending—especially those scaling up for the first time. Our technical support does not end at the dock door. We connect plant operations managers with those who have processed this product on ton-scale lines, sharing workarounds for common processing hiccups or advice for optimizing mixing and heating routines.

    End-user support has involved site visits, troubleshooting micro-clumping in high-speed mixers, and adjusting the granule profile to better fit automated feeders. All of these details—tracking, delivery advice, batch-to-batch consistency—emerge from direct engagement, not standard operating procedure paperwork alone.

    Conclusion

    As a true manufacturer of 2,2'-((4-((2-Hydroxyethyl)Amino)-3-Nitrophenyl)Imino)Bisethanol, our commitment goes beyond batch records and certificates. We carry customer challenges up to the lab bench and onto the production floor, solving problems at the source. The real difference is experience-backed knowledge, transparent engagement, and a relentless drive for practical, actual quality. Through ongoing dialogue with customers, iterative improvements, and constant investment in process innovation, we deliver more than a chemical—we deliver confidence.